#!@WHICHPERL@ -w
#
# $Id$
# $Log$
# Revision 1.1  2005/07/28 23:57:08  nadya
# Initial revision
#
#
# FILE: transfac2meme
# AUTHOR: William Stafford Noble and Timothy L. Bailey
# CREATE DATE: 4/22/99
# DESCRIPTION: Convert a Transfac matrix file to MEME output format. 

use strict;
use warnings;

use lib qw(@PERLLIBDIR@);

use MotifUtils qw(matrix_to_intern intern_to_meme read_background_file);

use Fcntl qw(O_RDONLY);
use Getopt::Long;
use Pod::Usage;

=head1 SYNOPSIS

transfac2meme [options] <matrix file>

 Options:
  -numbers                      use numbers instead of strings as motif names
  -use_acc                      use accession names instead of IDs
  -ids <idfile>                 keep any motifs listed in the file
  -species <name>               keep only motifs for this species
  -skip <ID>                    skip this ID (may be repeated)
  -bg <background file>         file with background frequencies of letters;
                                default: uniform background
  -pseudo <total pseudocounts>  add <total pseudocounts> times letter
                                background to each frequency; default: 0
  -logodds                      print log-odds matrix, too;
                                default: print frequency matrix only
  -url <website>                website for the motif; The ID (or accession) is
                                substituted for MOTIF_NAME; default: no url

 Converts a transfac matrix.dat file into MEME motifs.

 N.B. Dollar signs in TRANSFAC IDs are converted to underscores.

 Writes to standard output.

=cut

# Set option defaults
my $use_numbers = 0;
my $id_type = "ID";
my $species = "";
my %skips = ();
my $id_file = "";
my $bg_file;
my $pseudo_total = 0;
my $print_logodds = 0;
my $url_pattern = "";
my $matrix_file;

GetOptions("numbers" => \$use_numbers, "use_acc" => sub {$id_type = "AC"},
  "species=s" => \$species, "skip=s" => sub {$skips{$_[1]} = 1},
  "ids=s" => \$id_file, "bg=s" => \$bg_file, "logodds" => \$print_logodds, 
  "pseudo=f" => \$pseudo_total, "url=s" => \$url_pattern) or pod2usage(2);
#check matrix file
pod2usage("A matrix file must be specified for the conversion.") unless @ARGV;
$matrix_file = shift(@ARGV);
pod2usage("Only one matrix file may be specified for the conversion.") if @ARGV;
#check pseudo total
pod2usage("Option -pseudo must have a positive value.") if ($pseudo_total < 0);

# read the background file
my %bg = &read_background_file(1, $bg_file);

my $line;

# Store the target IDs.
my %id_list = ();
if ($id_file) {
  my $fp;
  sysopen($fp, $id_file, O_RDONLY) || die("Can't open \"$id_file\".");
  while ($line = <$fp>) {
    chomp($line);
    my @words = split(' ', $line);
    foreach my $word (@words) {
      $id_list{$word} = 1;
    }
  }
  close($fp);
}

# Open the matrix file for reading.
my $matrix_fp;
sysopen($matrix_fp, $matrix_file, O_RDONLY) || die("Can't open \"$matrix_file\".\n");

# Read the input file.
my $num_motifs = 0;
my $num_skipped = 0;
my $num_bad = 0;
while ($line = <$matrix_fp>) {
  chomp($line);
  # Split the line into type and everything else.
  my ($type, $data) = split(/\s+/, $line, 2);
  next unless $type;

  # Have we reached a new matrix?
  if ($type eq $id_type) {
    my $matrix_name;
    chomp($matrix_name = $data);
    $matrix_name =~ tr/\$/_/;

    # Read to the beginning of the motif.
    my $this_species = "";
    my $this_name = "";
    my $this_descr = "";

    # Old versions of TRANSFAC use pee-zero; new use pee-oh.
    while (($type ne "PO") && ($type ne "P0")) {
      $line = <$matrix_fp>;
      if (! defined($line)) {
        die ("Can't find PO line for TRANSFAC matrix $matrix_name.\n");
      }
      chomp($line);
      ($type, $data) = split(/\s+/, $line, 2);

      # Store the species line.
      if ($type eq "BF") { $this_species .= $data . "\n"; }

      # Store the name line; replace whitespace with "_".
      if ($type eq "NA") { $this_name = $data; $this_name =~ s/\s+/_/g; }

      # Store the description line.
      if ($type eq "DE") { $this_descr = $data; }
    }

    # Read the motif.
    my $matrix = "";
    while () {
      $line = <$matrix_fp>;
      die ("Can't find `XX' line for TRANSFAC matrix $matrix_name.\n") unless $line;
      chomp($line);

      ($type, $data) = split(/\s+/, $line, 2);

      # Look for the end of the motif.
      last if (($type eq "XX") || ($type eq "//"));

      # trim the IUPAC code off the end
      $data =~ s/\w$//;

      # append to the matrix
      $matrix .= $data . "\n";
    }

    ###### Decide whether to print the motif.

    # If no criteria are given, then print it.
    my $print_it = 1;

    # If we were given a list,
    if ($id_file) {
      #  was this matrix in the list?
      $print_it = 0 unless defined($id_list{$matrix_name});
    }

    # If we were given a species.
    if ($species ne "") {
      # is this the right species?
      $print_it = 0 unless ($this_species =~ m/$species/);
      if ($this_species eq "") {
        print(STDERR "Warning: No species given for $matrix_name.\n");
      }
    }

    # Were we explicitly asked to skip this one?
    if ($skips{$matrix_name}) {
      $print_it = 0;
    }
    # Print the motif.
    if ($print_it) {
      my $url = $url_pattern;
      $url =~ s/MOTIF_NAME/$matrix_name/g;
      my $name = ($use_numbers ? $num_motifs + 1 : $matrix_name);
      my $alt = ($use_numbers ? $matrix_name : $this_name);
      my ($motif, $errors) = matrix_to_intern(\%bg, $matrix, 'row', 1, $pseudo_total, id => $name, alt => $alt, url => $url);
      print(STDERR join("\n", @{$errors}), "\n") if @{$errors};
      $num_bad++ unless $motif;
      print intern_to_meme($motif, $print_logodds, 1, !($num_motifs++)) if $motif;
    } else {
      $num_skipped++;
    }
  }
}
print(STDERR "Converted $num_motifs motifs.\n");
print(STDERR "Skipped $num_skipped motifs.\n");
print(STDERR "$num_bad conversion errors.\n");

close($matrix_fp);