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The amplification of target DNA is an important stage in current sequencing methods. DNA can be replicated using bacterial cultures (cloning), or via the polymerase chain reaction. PCR is often preferred due to the speed and high through-put of PCR systems. This is accomplished by placing the DNA extractions in a specialized chemical environment and cycling them through a specific temperature regime. A heat-activated DNA polymerase replicates the target DNA sequence with each cycle. |
